河南农业科学2012，Vol.41Issue(2)：136-139,4.
热应激下中草药对鸡法氏囊、胸腺淋巴细胞组织学的影响
Effects of Chinese Herbal Medicine on the Histology of the Lymphocytes in Bursa of Fabricius and Thymus of Chickens under Heat Stress
摘要
Abstract
To valuate the immune mechanism of Chinese herbal medicine for heated-stress chickens, the histological changes of the central immune organs were observed. A total of 120 healthy 88-day-old Isa Brown commercial laying chickens were randomly divided into six groups,three experimental groups (High,medium and low dose groups) and three control groups (High temperature, room temperature and Vc (5 mg/d) groups). In the experimental groups chickens were fed with the high dose (7. 5 mg/d), the medium dose (5 mg/d), or the low dose (2. 5 mg/d) of Chinese herbal medicine. On Days 88,91,95,and 97,the changes of lymphocyte in bursa of fabri-cius and thymus of chicken were examined. The bursa of fabricius and thymus tissue samples were processed routinely for paraffin embedding technique and stained with haematoxylin and eosin (H. E). The results showed that on Day 97,the lymphocytes in bursa of fabricius added with 7. 5 mg/d Chinese herbal medicine was significantly increased than that of the high temperature control group (P<0. 01), which are 84. 33 and 69. 81, respectively. The lymphocytes in bursa of fabricius of chicken added with 5 mg/d Chinese herbal medicine was 81. 61, significantly increased than that of high temperature control group (P<0. 05). The lymphocytes in thymus of the high dose and the medium dose groups were 95. 85 and 91. 40,higher than that of the high temperature control group (P<0. 01). This indicated that adding 7. 5 mg/d Chinese herbal medicine for heated-stress chickens significantly increased lymphocytes in bursa of fabricius and thymus of chicken and the higher concentration the better,which implies that the Chinese herbal medicine is useful for easing the effects of heat stress.关键词
中草药/鸡/法氏囊/胸腺/淋巴细胞/热应激Key words
Chinese herbal medicine/ chicks/ bursa of fabricius/ thymus/ lymphocytes/ heat stress分类
农业科技引用本文复制引用
刘会茹,沈萍,杨彩然,史秋梅,董淑珍,杨光,左天衡..热应激下中草药对鸡法氏囊、胸腺淋巴细胞组织学的影响[J].河南农业科学,2012,41(2):136-139,4.基金项目
河北省科技厅资助项目(07220401D) （07220401D）
石家庄市科技发展计划项目(08150132A) （08150132A）
