核农学报2011，Vol.25Issue(4)：639-644,6.
14份小麦种质资源抗麦长管蚜遗传多样性的SSR分析
GENETIC DIVERSITY OF WHEAT GERMPLASM RESISTANCE TO THE APHID（Sitobion avenae F.） BY SSR MARKERS
摘要
Abstract
The objective of this paper is to study the genetic diversity of wheat germplasm resistance to the grain aphid Sitobion avenae,providing theoretical basis and technical support for efficient breeding of environment-friendly wheat by SSR markers.A total of 32 pairs specific primers were selected from 175 pairs SSR primers distributing on A,B and D homologous chromosomes.The results showed that group D had higher genetic diversity than other groups.Among all the 21 pairs of chromosomes,genetic diversity was highest on chromosome 7D,and lowest on chromosomes 7A.A total of 255 bands were amplified with 32 pairs of primers among 14 wheat germplasm,of which 244 bands were polymorphic. Each pair of primers amplified 3 to 13 bands with an average of 6.8 bands.The percentage of polymorphic band was ranged from 40% to 100%.Nei＇s Genetic diversity and Shannon information index were in the range of 0.3264～0.4243 and 0.5078～0.6154,respectively.Cluster analysis showed that 14 wheat accessions could be divided into 3 groups by SSR,which were consistent with that of traditional classification,i.e.the germplasm resources with the same resistance were clustered in the same group.It is suggested that the genetic diversity of resistant to aphid in wheat germplasms could be efficiently assessed by SSR markers so that the SSR molecular markers could be effectively used in molecular breeding of new varieties resistant to aphid.关键词
小麦种质资源/SSR分子标记/麦长管蚜/遗传多样性Key words
wheat germplasms/SSR marker/Sitobion avenae F./genetic diversity分类
农业科技引用本文复制引用
王春平,罗坤,赵惠燕,张改生,李丹,高欢欢,邓明,刘兰..14份小麦种质资源抗麦长管蚜遗传多样性的SSR分析[J].核农学报,2011,25(4):639-644,6.基金项目
国家863计划重大专项 （）
陕西省13115科技创新工程重大科技专项 （）
国家杨凌农业生物技术育种中心专项基金 （）
西北农林科技大学拔尖人才支持计划项目 （）
中德农业合作资助项目[2008/2009（04）]共同资助 （）
