核农学报2011，Vol.25Issue(4)：652-656,5.
快中子辐照对花生胚小叶体细胞胚胎发生的影响
EFFECT OF NEUTRON IRRADIATION ON SOMATIC EMBRYOGENESIS FROM EMBRYONIC LEAFLETS OF PEANUT（Arachis hypogaea L.）
摘要
Abstract
The effect of neutron irradiation on somatic embryogenesis in embryonic leaflet culture of peanut（Arachis hypogaea L.）was studied.The embryonic leaflets separated from seeds of peanut cultivar Luhua 11,which were irradiated at dosages of 0,9.7,14.0 and 18.0Gy,were incubated on MSB5 medium containing 10.0mg/L 2,4-D to induce somatic embryos.After 4 weeks culture,the leaflets were transferred to MSB5 medium supplemented with 4.0mg/L BAP.The embryonic leaflets from the unirradiated seeds formed somatic embryos directly on induction medium containing 10.0mg/L 2,4-D.However,after irradiation most of embryonic leaflets first formed calli,when transferred to MSB5 medium containing 4.0mg/L BAP,some of them formed somatic embryos.Only a few of them formed somatic embryos directly on induction medium containing 10.0mg/L 2,4-D.The somatic embryo formation rates varied with different dosages of neutron irradiation.The percentage of somatic embryo formation declined with the increase of neutron irradiation dosages.When the irradiation dosages were increased from 9.7Gy to 14.0 and 18.0Gy,the percentage of somatic embryo formation declined from about 55% to 40% and 30%,respectively.The unirradiated embryonic leaflets gave a higher rate of somatic embryo formation（above 80%）.The results showed that neutron irradiation was efficient to induce genetic mutation in peanut and the optimum dosage was about 14.0Gy.关键词
花生/快中子辐照/离体诱变/体细胞胚胎发生Key words
peanut（Arachis hypogaea L.）/fast neutron irradiation/in vitro mutation/somatic embryogenesis分类
农业科技引用本文复制引用
王莉莉,赵明霞,乔利仙,王晶珊,隋炯明,刘录祥..快中子辐照对花生胚小叶体细胞胚胎发生的影响[J].核农学报,2011,25(4):652-656,5.基金项目
国家自然基金项目 （）
国家公益性行业科研专项 （）
农业部农业公益性行业科研专项 （）
