核农学报2011，Vol.25Issue(4)：719-723,5.
紫外线对杏鲍菇原生质体的诱变作用
UV-MUTAGENESIS OF PROTOPLASTS OF Pleurotus eryngii
摘要
Abstract
The UV-mutagenesis of protoplast was conducted on Pleurotus eryngii strain PL7.The results showed that the lethality rate of protoplast reached to 74% by using a 20W germicidal lamp with emitting wavelength of 254nm under irradiation condition of vertical distance of 30cm from lamp to culture media for an irradiation duration of 90s.Twenty mutants were selected from 234 regenerating colonies by antagonistic test.6 isolates exhibiting 11.0%～17.8% increase of colony growth speed,19.3%～32.4% increase of mycelium biomass in liquid medium,and new Rep-PCR genetypic pattern were screened from the 20 mutants.The assay of mushroom cultivation revealed that the 6 isolates shortened the overgrowth time of mycelium on culture medium by 3～6 days,advanced the appearance time of mushroom anlage by 5～8 days,and increased the mushroom yield of the first harvest by 12.5%～40.6% and the total biological efficiency by 11.1%～32.4%.The results indicated that the protoplast UV-mutagenesis was a better approach for novel high-yield isolate selection and strain improvement of edible fungi.关键词
杏鲍菇/原生质体/紫外诱变Key words
Pleurotus eryngii/protoplast/UV-mutagenesis分类
农业科技引用本文复制引用
刘海英,张运峰,范永山,李科南,刘先拉..紫外线对杏鲍菇原生质体的诱变作用[J].核农学报,2011,25(4):719-723,5.基金项目
河北省科技支撑计划项目 （）
河北省自然科学基金项目 （）
