核农学报2011，Vol.25Issue(5)：910-915,921,7.
茶树生长素抑制蛋白基因CsARP1的克隆与表达分析
CLONING AND EXPRESSION ANALYSIS OF AUXIN-REPRESSED PROTEIN GENE CsARP1 IN TEA PLANT（Camellia sinensis）
摘要
Abstract
A 3′-end gene fragment of auxin-repressed protein gene（ARP） was screened from the tea plant dormant bud suppression subtractive hybridization（SSH） library,its full-length cDNA sequence was cloned through rapid amplification of cDNA ends（RACE）,and its relative expression quantity in different stages of dormant buds was analyzed by real-time fluorescence quantitative PCR.The full length of the auxin-repressed protein gene,named CsARP1,was 711bp（GenBank accession No.HQ225758） and contained a 357bp open reading frame（ORF） encoding a 118 amino acid residues,and its 3′ untranslated region was an obvious polyadenylation signal.The deduced protein molecular weight was 12.82kD and its theoretical isoelectric point was 9.57.Sequence alignment of the deduced amino acids of CsARP1revealed a high degree of similarity with other members of plant ARP and had a typical domain characteristic.The results of real-time quantitative PCR showed that the CsARP1gene was expressed at a higher level in dormant buds than in sprouting buds.It suggests that the expression of CsARP1gene is correlated to the bud dormancy transition.关键词
茶树/生长素抑制蛋白基因CsARP1/RACE/序列分析/实时荧光定量PCRKey words
tea plant（Camellia sinensis）/auxin-repressed protein gene（CsARP1）/RACE/sequence analysis/real-time fluorescence quantitative PCR分类
农业科技引用本文复制引用
王新超,马春雷,杨亚军,姚明哲,金基强..茶树生长素抑制蛋白基因CsARP1的克隆与表达分析[J].核农学报,2011,25(5):910-915,921,7.基金项目
国家自然科学基金项目 （）
现代农业（茶叶）产业技术体系项目 （）
