核农学报2011，Vol.25Issue(5)：916-921,6.
十字花科植物C2H2型锌指蛋白新基因BcMF20同源序列克隆与进化分析
CLONING AND EVOLUTIONARY ANALYSIS OF HOMOLOGOUS SEQUENCES OF A NOVEL GENE ENCODING C2H2 ZINC FINGER PROTEIN IN CRUCIFERAE
摘要
Abstract
In order to elucidate categorization evolvement of C2H2 zinc finger protein,and clarify mechanism on C2H2 zinc finger protein in pollen development among plants at molecular level,the genes encoding C2H2 zinc finger protein analogues from 18 species of genera Brassicaand Raphanusin Cuciferae were obtained by PCR strategy using specific primers designed from the full length of BcMF20,a putative gene encoding C2H2 zinc finger protein which was related to the male sterility.The phylogenetic relationships of these species belonging to the family Cruciferae were investigated through comparison of the sequences.Homologous sequences of BcMF20comparison indicated that the similarities among the genes at nucleotide and amino acid levels were 86.9% ~ 100% and 77.6%～100%,respectively.In the zinc finger regions of homologous sequences,the amino acid sequences were identical.These results showed that the BcMF20was relative conservation in evolution in Cruciferae,and BcMF20may play an important role in pollen development.关键词
十字花科/C2H2锌指蛋白/BcMF20/克隆/系统发育Key words
Cruciferae/C2H2 zinc finger protein/BcMF20/isolated/phylogenic development分类
生物科学引用本文复制引用
韩莹琰,张爱红,范双喜,曹家树..十字花科植物C2H2型锌指蛋白新基因BcMF20同源序列克隆与进化分析[J].核农学报,2011,25(5):916-921,6.基金项目
国家自然科学基金 （）
浙江省重大科技项目 （）
北京市教委科技项目 （）
北京市科技新星项目 （）
