核农学报2011，Vol.25Issue(5)：939-944,6.
牡丹PsMADS5基因的克隆、表达及载体构建
CLONING,EXPRESSION AND VECTOR CONSTRUCTION OF PsMADS5 GENE IN TREE PEONY
摘要
Abstract
In this work,a full-length cDNA sequence of SEPALATAgene was obtained from petals of tree peony（Paeonia suffruticosa L.cv.Zhao Fen）using RT-PCR and RACE,named PsMADS5（GenBank accession No.HQ449569）.The full length of PsMADS5cDNA is 1222bp in length,containing a 5′-untranslated region（5′-UTR） of 190bp,a 3′-UTR of 302bp,and an opening reading frame（ORF） of 732bp encoding a 243 predicted amino acids.Subsequently,sequence comparison and phylogenetic analysis revealed that PsMADS5shared more than 83% homology with Vitis vinefera,belonged to SEPsubfamily of MADS family.However,relative real-time PCR analysis indicated that PsMADS5showed the highest transcript abundance in petals,moderate levels in carpels,low levels in sepals and the lowest levels in stamens.Construction of sense and antisense plant expression vector laid a foundation for flower type modification and variety innovation.关键词
牡丹/基因克隆/表达/载体构建Key words
tree peony/gene cloning/expression/construction of vector分类
农业科技引用本文复制引用
任磊,王雁,周琳,彭镇华..牡丹PsMADS5基因的克隆、表达及载体构建[J].核农学报,2011,25(5):939-944,6.基金项目
国家863项目 （）
国家林业局948项目 （）
