核农学报2011，Vol.25Issue(5)：945-950,6.
芥菜Rubisco小亚基的基因克隆及其在芜菁花叶病毒侵染后的表达分析
CLONING OF RUBISCO SMALL SUBUNIT GENE FROM MUSTARD AND ANALYSIS OF ITS EXPRESSION IN RESPONSE TO TURNIP MOSAIC VIRUS INFECTION
摘要
Abstract
We cloned the cDNA encoding the Rubisco small subunit of leafy mustard and studied its expression after Turnip mosaic virus（TuMV） infection.The result showed that the full-length cDNA of Rubisco small subunit gene was 842bp and contained an open reading frame（ORF） encoding 181 amino acids,which had two α-helixes,four β-sheets,and some conserved residues.With the accumulation of CP in upper systemic leaves after TuMV infection,mustard growth was impaired.At the same time,expression of Rubisco small subunit was increased before 6 days of inoculation and then gradually decreased,which suggested that the Rubisco small subunit participated in the response of mustard to TuMV infection.关键词
芥菜/Rubisco小亚基/抗病性/叶绿素荧光/TuMVKey words
mustard/Rubisco/virus resistance/chlorophyll fluorescence/Turnip mosaic virus分类
农业科技引用本文复制引用
朱磊,杨景华,张明方..芥菜Rubisco小亚基的基因克隆及其在芜菁花叶病毒侵染后的表达分析[J].核农学报,2011,25(5):945-950,6.基金项目
教育部新世纪优秀人才支撑计划 （）
