核农学报2011，Vol.25Issue(5)：1052-1056,5.
聚乙二醇化重组新型人白细胞介素-6的药代动力学研究
PHARMACOKINETICS OF PEGYLATED RECOMBINAN NEW HUMAN INTERLEUKIN-6（PEG-rhIL-6）
摘要
Abstract
In this study,the newly developed Pegylated recombinan human Interleukin-6（PEG-rhIL-6） was investigated.With ^125I labeled PEG-rhIL-6 by two-phase chloramine-T method.Thirty of the SD rats were randomly divided into 6 groups（group 1,2,3,4,5 and 6,respectively）,with 5 animals（3 males and 2 females） each group.Group 1,2,and 3 were injected subcutaneously with125I-PEG-rhIL-6 at the dose of 40,20 and 3μg/kg,respectively.Group 4,5,and 6 were injected intravenously with125I-PEG-rhIL-6 with the same dose level,respectively.Blood samples were collected at different time intervals after injection.The plasma concentration（ng/ml）of125I-PEG-rhIL-6 were determined by125I isotope tracing method and then assessed with 3P87 software to obtain the pharmacokinetic parameters.Results clearly showed that 1/2ke（20.41,21.53,19.77）and t1/2ka（10.44,11.26,11.37）of group1-3 are higher number than t1/2β（17.54,18.78,17.81）and t1/2α（1.93,1.60,1.78）of grou4-6,The pharmacological activity of125I-PEG-rhIL-6 maintain longer time by subcutaneous injection;The pharmacokinetic of V/Fc after sc（694.96ml/kg）is higher than those after iv（115.26ml/kg）,which showed that the excretion speed of125I-PEG-rhIL-6 given by sc was slowly.The above results obtained indicated that the pharmacokinetic characteristics of125I-PEG-rhIL-6 would provide important information for clinical application and the subcutaneous injection is better.关键词
白细胞介素-6/药代动力学/组织分布/同位素示踪Key words
interleukin-6/pharmacokinetics/distribution/isotope tracing分类
农业科技引用本文复制引用
王振华,汪淑芳,曹晓涵,杜小刚,陈志俞,曾宪垠..聚乙二醇化重组新型人白细胞介素-6的药代动力学研究[J].核农学报,2011,25(5):1052-1056,5.基金项目
“教育部长江学者和创新团队发展计划”创新团队项目 （）
