核农学报2011，Vol.25Issue(6)：1088-1093,1190,7.
水稻光叶性状基因gl1的精细定位与候选基因分析
FINE MAPPING AND CANDIDATE GENE ANALYSIS OF GLABROUS LEAF AND HULL GENE（gl1） IN RICE（Oryza sativa L.）
摘要
Abstract
Glabrous rice is a kind of rice that is hairless on the surface of its leaves and grains.It is the main grown cultivar in the southern region of the United States and in many African countries.It has been proved that the glabrous trait is controlled by the recessive gene gl1 on chromosome 5.This study aimed to fine map and identify candidate genes for the eventual cloning of gl1 and for further analysis of trichome morphogenesis and regulation.One segregating population was established by crossing a glabrous tropic Japonica rice cultivar（AP9） and non-glabrous indica cultivar（Zhehui 7954）,and microsatellite（SSR） markers were identified around the region of RFLP markers which were previously identified to be linked with gl1.These SSR markers were used for linkage analysis of the AP9×Zhehui 7952 population and proved that gl1 was linked to SSR marker RM17990.Further analysis using more new SSR markers and insertion/deletion（InDel） markers mapped gl1 to the region between RM17759 and RM19831（physical interval 538kb~1.649Mb）.To further delimit gl1,capillary electrophoresis and tetra-primer amplification refractory mutation system PCR markers（referred as ASPCR markers hereafter） were used for SNP analysis and bracketed gl1 to 770.5￣977.1kb.This region was further narrowed down to a 6.1kb segment between 971~977.1kb by combining mapping results of this experiment and a newly published mapping report.After sequencing all four annotated genes within the 6.1kb fragment,only one SNP was discovered between glabrous rice and pubescent rice in the 5＇-end untranslated region of LOC_Os05g02750,hence LOC_Os05g02750 is very likely to be the gene controlling glabrous traits in rice.关键词
水稻/光叶/gl1/毛状体/精细定位Key words
rice（Oryza sativa L.）/glabrous leaf/gl1/trichome/fine mapping分类
农业科技引用本文复制引用
洪隽,王启钊,富昊伟,吴殿星,舒庆尧..水稻光叶性状基因gl1的精细定位与候选基因分析[J].核农学报,2011,25(6):1088-1093,1190,7.基金项目
国家科技支撑项目 （）
