核农学报2011，Vol.25Issue(6)：1107-1116,10.
玉米海藻糖含量测定及其合成酶（TPS）基因序列分析
TEST OF TREHALOSE CONTENT AND SEQUENCE ANALYSIS OF TREHALOSEPHOSPHATE SYNTHASE GENE（TPS） IN MAIZE
摘要
Abstract
The trehalose exists abroad in natural organisms.It is not only providing energy reservior for metabolism in general condition,but also resisting hurt from adversity stress such as drought,heat,cold and so on by accumulation excessively.So,studying on the trehalose accumulating mechanism and key enzyme in its synthesis approach has been giving more and more attentions.The leaf trehalose contents of maize inbred lines under different drought treatments were tested by high performance liquid chromatography（HPLC） in this study,Selaginella pulvinata（Hook.et Grev） Maxim with in better water.The results showed that trehalose was not tested in all samples of maize,and 10.31mg/g drought weight in Selaginella pulvinata（Hook.et Grev） Maxim was measured.All 3055bp cDNA sequence of trehalose-phosphate synthase gene（TPS） was cloned from maize inbred line ＂18-599 Bai＂ by combining electronic cloning method and RT-PCR.This sequence named as ZmTPS and logging in GenBank as EU659122.Comparing and analysing with other species＇ amino acid sequences of TPS,some of conservative sequences was discoverred in maize TPS,but existed partial conservative sequences,which was same as other plant species with low activity of TPS enzyme.It is estimated that the differences between TPS of maize and other species may result in too low content trehalose in maize to be tested by HPLC.关键词
玉米/海藻糖/高效液相色谱/海藻糖-6-磷酸合成酶/序列分析Key words
maize/trehalose/HPLC/TPS/sequence analysis分类
农业科技引用本文复制引用
付凤玲,阎雨,刘卫国,李晚忱..玉米海藻糖含量测定及其合成酶（TPS）基因序列分析[J].核农学报,2011,25(6):1107-1116,10.基金项目
国家自然科学基金（31071433） （）
抗逆转基因专项 （）
