核农学报2011，Vol.25Issue(6)：1148-1156,9.
基于悬浮细胞培养的半夏多倍体诱导研究：Ⅱ.单细胞水平COLO诱导及多倍体植株再生
POLYPLOID INDUCED OF Pinellia ternata BASED ON SUSPENDED CELL CULTURE：Ⅱ.INDUCTION OF POLYPLOID CELL BASED ON SINGLE-CELLED LEVEL VIA COLO AND REGENERATION OF POLYPLOID PLANTS
摘要
Abstract
Based on mature and efficient single-celled suspension cultivation and plant regeneration system,suspended cells were used as target material,key factors of polyploid induction via COLO,were studied in this paper.Homozygous polyploid plants were obtained via differentiation and regeneration culture after polyploid cell was obtained.The results showed that better induction efficiency of polyploid cell was obtained after suspended cells was treated with 0.05~0.10mg/ml COLO.Ratio of polyploid cell was about 74.20%.Eleven homozygous polyploid plants of Pinellia ternata was obtained finally and its cell chromosome number of root tip was 104 which was two times of control.Visual mutagenic rate was 14.07%~46.05% and induction rate of polyploid plants was 3.85%~29.76%,and rate of chimera was 12.88%~17.65%.The results proved that it was a viable and effective polyploid induction approach which suspended cell was induced into polyploidy cell via COLO and then differentiation and regeneration into homozygous polyploids.关键词
半夏/悬浮细胞/单细胞水平/秋水仙素/多倍体/胚胎发生/植株再生Key words
Pinellia ternata Briet./suspended cells/single-celled level/COLO/polyploid/embryogeny/plant regeneration分类
农业科技引用本文复制引用
欧巧明,岳春玲,厚毅清,崔文娟,郑秀芳,罗俊杰,陈玉梁..基于悬浮细胞培养的半夏多倍体诱导研究：Ⅱ.单细胞水平COLO诱导及多倍体植株再生[J].核农学报,2011,25(6):1148-1156,9.基金项目
国家转基因生物新品种培育重大专项 （）
甘肃省农科院农业科技创新专项（2011GAAS06-11）,甘肃省农科院农业科技创新专项 （）
甘肃省自然科学基金项目 （）
