核农学报2012，Vol.26Issue(1)：22-27,6.
利用SRAP分子标记评价小麦三雌蕊近等基因系的遗传背景
EVALUATION ON THE GENETIC BACKGROUND OF WHEAT NEAR ISOGENTIC LINES FOR THREE PISTILS CHARACTER BY SRAP MARKERS
摘要
Abstract
For common wheat line, three pistils （TP）, is a valuable mutant trait for wheat breeding, because it could potentially increase the number of gains per spike. In this study, the material with 3 pistils was used as donor parent, and Chinese spring, Chuanmai 28, Mianyang 29 and Neimai 9 were used as recurrent male parents. After successive backcrossing for 7 generations and then self-crossing for 4 generations, 4 near isogentic lines （NILs）, CSTP, CM28TP, MY29TP and NM9TP were bred. Genetic similarity and genetic distance of 4 near isogentic lines and their recurrent parents were compared and analyzed by SRAP marker. The results were summarized as follows ： （ 1 ） All the 128 pairs of SRAP primers amplified 978 bands, 120 pairs （93.8%） of primers produced polymorphic bands and 638 bands （65.2%） were polymorphic ; （2） The genetic similarity coefficient of CSTP, CM28TP, MY29TP and NM9TP with their recurrent parents were 0. 9346, 0. 9397, 0. 9070 and 0. 8732, respectively. （3） Cluster analysis revealed that CSTP and CM28TP had least difference with the recurrent parent, and was clustered into a small class with the similarity coefficient of 0. 93.关键词
小麦/三雌蕊性状/近等基因系/SRAPKey words
wheat/three pistils character/near isogentic lines/SRAP分类
农业科技引用本文复制引用
杨在君,彭正松,周永红,彭丽娟,魏淑红..利用SRAP分子标记评价小麦三雌蕊近等基因系的遗传背景[J].核农学报,2012,26(1):22-27,6.基金项目
国家自然科学基金项目 （）
