核农学报2012，Vol.26Issue(1)：54-59,67,7.
雄性不育烟草atp6基因育性相关生物信息学分析
BIOINFORMATICS ANALYSIS OF apt6 GENE RELATED TO TOBACCO MALE STERILITY
摘要
Abstract
Mitochondrial gene atp6 had close relation with cytoplasmic male sterility （CMS） of various plants. To understand the potential function of apt6 gene on tobacco （Nicotiana tabacum） CMS, we cloned atp6 gene from two CMS lines and their maintainer lines. Sequencing results showed six different bases in atp6 genes for two lines. Bioinformatics analysis of their predicted protein revealed that the six base differences would lead to the differences of four predicted residues and the hydrophilic/hydrophobic profiles in corresponding regions. The predicted proteins from CMS lines presented a changing trend of increasing periodic structure （mainly being a-helix） but decreasing aperiodic structure （mainly being coil） in the secondary structures and domains. The above information indicated that the atp6 base differences of CMS lines could lead to the change in spatial structure of their coding subunits. It is presumed that structural differences in subunit 6, especially in its domain might be a potential factor that disturbed the function of FoF1-ATP synthetase in mitochondrion and thus became one of the possible causes of tobacco CMS.关键词
烟草/细胞质雄性不育/atp6基因/育性/生物信息学Key words
tobacco/cytoplasmic male sterility/atp6 gene/fertility/bioinformatics分类
农业科技引用本文复制引用
周玮,刘齐元,朱腾义,范东东,朱肖文,程元强..雄性不育烟草atp6基因育性相关生物信息学分析[J].核农学报,2012,26(1):54-59,67,7.基金项目
国家自然科学基金项目（30660099）,高等学校博士学科点专项科研基金新教师类资助课题 （）
