中医药导报2012，Vol.18Issue(1)：6-9,11,5.
截断逆挽方降低慢加急性肝衰竭大鼠死亡率的机制探讨
Truncated inverse pull side to reduce the mechanism of the slow increase of the mortality of acute liver failure in rats
摘要
Abstract
Objective: To investigate the truncated inverse pull side to reduce the acute on chronic liver failure in rats 24 h, mortality, and increase the survival time of the mechanism of action. Method: Wistar rats of SPF grade 150, divided into normal group, model group, the chinese medicines board. Human serum albumin in rat liver cirrhosis model, traditional chinese medicine group was given the truncated inverse pull side gavage for 3 days. Given herbal group and model group DGalN.400 mg/Kg plus LPS-100 μg/Kg intraperitoneal joint injection. After injection model group and the group were divided into 4 groups: executed a set of 4, 8, 12 h after each anesthesia of liver tissue HE staining and in situ detection of apoptosis (TUNEL) techniques, and calculation of the liver cells the apoptotic index (AI); I observed 24 h mortality and record survival time. Results: Compared with normal group, model group at all time points HE staining showed intrahepatic pseudolobule widely formation of regenerative nodules within the liver cell necrosis, inflammatory infiltration and hemorrhage significantly, 12 h,most significantly, the performance for large pieces or sub-chunk necrosis; TUNEL assay to see a large number of apoptotic bodies and apoptotic cells, the AI were significantly increased (P<0.01); compared with model group, HE staining of the TCM group the corresponding time point of necrosis and hemorrhage significantly reduced inflammatory cell infiltration reduced;TUNEL assay medicine group for 4 h, 8 h, apoptotic cells decreased 12 h, the necrotic area was narrow, the AI decreased (P<0.05). TCM group than in model group 24 h mortality, and prolonged survival. Conclusion: The truncated inverse pull side reduce the ACLF rats 24 h mortality, prolong the survival time of the mechanism may block the cellular processes of hepatic apoptosis, or delay the apoptosis of liver cell transformation related to the death.关键词
截断逆挽方/慢加急性肝衰竭/24h死亡率/生存时间Key words
Truncated inverse pull side/ Acute on chronic liver failure/ For 24 h mortality/ Survival time分类
医药卫生引用本文复制引用
吴文秀,张秋云,崔利娟,高连印,杜宇琼,陈煜..截断逆挽方降低慢加急性肝衰竭大鼠死亡率的机制探讨[J].中医药导报,2012,18(1):6-9,11,5.基金项目
首都中医药与护理研究专项重点课题(NO.10ZYH02),北京市自然基金面上项目(NO.7112017) （NO.10ZYH02）
