中医药导报2012，Vol.18Issue(1)：12-14,3.
康莱特注射液对免疫低下小鼠的免疫功能及NF-κB蛋白表达的影响
Effect of Kanglaite injection on immune function and expression of NF-κB of immunosuppressed mice
摘要
Abstract
Objective: To investigate the effect of kanglaite injection (KLT) on immunological function and expression of NF-κB in immunosuppressed mouse model. Methods:Forty C57BL/6 mice were divided randomly into the high dose(25 mL/kg),low dose (6.25 mL/kg) of KLT groups, model group and control group(n=10). All mice except that in control group were made into immunosuppressed models induced by CsA injection. High dose and low dose groups of KLT were treated with KLT at a dose of 25 mL/kg or 6.25 mL/kg by I.p. Injection. All mice were killed after 7 days. Spleens were removed and calculated spleen index, expression of IL-2 in spleen cells was detected using ELISA kit, western blot was used to assay protein expression of NF-κB and IκBα in spleens. Results:The spleen index of mice in KLT high dose and low dose groups were significantly higher than model group and control group (P <0.05). Comparing with model group, IL-2 expression of KLT high dose and low dose groups increased significantly (P<0.05). The protein expression of NF-κB in the nucleus increased and descended in the cytoplasm for IκBα in KLT high dose and low dose groups compared with model group. Conclusion:KLT could enhance immunological function by effecting spleen index, expression of IL-2, NF-κB and IκBα in immunosuppression mice models.关键词
康莱特注射液/免疫低下/IL-2/NF-κB/IκBαKey words
Kanglaite injection/ Low immunity/ IL-2/ NF-κB/ IκBα分类
医药卫生引用本文复制引用
吴岩,王彧杰,潘沛,王蓉,马维娜,原永芳..康莱特注射液对免疫低下小鼠的免疫功能及NF-κB蛋白表达的影响[J].中医药导报,2012,18(1):12-14,3.基金项目
上海市卫生局科研基金项目(2009103) （2009103）
