华侨大学学报（自然科学版）2011，Vol.32Issue(6)：672-675,4.
双水相萃取法富集分离地木耳中的藻蓝蛋白
Aqueous Two-Phase Extraction for Enrichment and Separation of Phycocyanin from Nostoc
摘要
Abstract
Enrichment and separation of phycocyanin from nostoc cell homogenates was studied under polyethylene glycol (PEG 4000)/sulphate aqueous two-phase system by one step extraction. The effects of time, type and concentration of sulphate, the concentration of PEG 4000, ionic strength and pH value on the extraction behavior were investigated respectively. The optimized extraction rate of phycocyanin from nostoc was 95. 2% and reached the purity of 4. 8, and the optimum conditions for extraction were extraction time of 30 min, Na2SO4 mass fraction of 15%, PEG 4000 mass fraction of 12%, KCl mass fraction of 1% with pH value of 3. 0.关键词
地木耳/藻蓝蛋白/聚乙二醇/双水相体系/萃取Key words
nostoc/phycocyanin/polyethylene glycol/aqueous two-phase system/extraction分类
化学化工引用本文复制引用
甘林火..双水相萃取法富集分离地木耳中的藻蓝蛋白[J].华侨大学学报（自然科学版）,2011,32(6):672-675,4.基金项目
华侨大学科研基金资助项目(08X0211*) （08X0211*）
