花生学报2011,Vol.40Issue(4):1-8,26,9.
花生中两个CBF-like基因全长的克隆与序列分析
Clone and Sequence Analysis of Two CBF-like Genes in Peanut
摘要
Abstract
The function of DREB/CBF transcript factors had been extensively studied in Arabidopsis and rice. However, no researches had been done in peanut about this kind of gene. In this study, we found 15 ESTs which may encode DREB/CBF proteins from the cDNA library of peanut using the Bioedit software. 5' fragments of 2 ESTs were unknown and we amplified the 5'-end of these two genes through 5'--RACE RT--PCR. The sequences of the two genes were also submitted to Gen- Bank and analyzed after the whole sequences were cloned.关键词
CBF—like基因/花生/基因克隆/序列分析Key words
CBF-like gene/peanut (Arachis hypogaea L. )/gene clone/sequence analysis分类
农业科技引用本文复制引用
陈娜,禹山林,程果,胡东青,杨庆利,迟晓元,潘丽娟,杨珍,陈明娜,和亚男..花生中两个CBF-like基因全长的克隆与序列分析[J].花生学报,2011,40(4):1-8,26,9.基金项目
山东省自然科学基金项目 ()
国家自然科学基金项目 ()