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花生中两个CBF-like基因全长的克隆与序列分析

陈娜 禹山林 程果 胡东青 杨庆利 迟晓元 潘丽娟 杨珍 陈明娜 和亚男

花生学报2011,Vol.40Issue(4):1-8,26,9.
花生学报2011,Vol.40Issue(4):1-8,26,9.

花生中两个CBF-like基因全长的克隆与序列分析

Clone and Sequence Analysis of Two CBF-like Genes in Peanut

陈娜 1禹山林 1程果 2胡东青 2杨庆利 1迟晓元 1潘丽娟 1杨珍 1陈明娜 1和亚男1

作者信息

  • 1. 山东省花生研究所,山东青岛266100
  • 2. 青岛市出入境检验检疫局,山东青岛266001
  • 折叠

摘要

Abstract

The function of DREB/CBF transcript factors had been extensively studied in Arabidopsis and rice. However, no researches had been done in peanut about this kind of gene. In this study, we found 15 ESTs which may encode DREB/CBF proteins from the cDNA library of peanut using the Bioedit software. 5' fragments of 2 ESTs were unknown and we amplified the 5'-end of these two genes through 5'--RACE RT--PCR. The sequences of the two genes were also submitted to Gen- Bank and analyzed after the whole sequences were cloned.

关键词

CBF—like基因/花生/基因克隆/序列分析

Key words

CBF-like gene/peanut (Arachis hypogaea L. )/gene clone/sequence analysis

分类

农业科技

引用本文复制引用

陈娜,禹山林,程果,胡东青,杨庆利,迟晓元,潘丽娟,杨珍,陈明娜,和亚男..花生中两个CBF-like基因全长的克隆与序列分析[J].花生学报,2011,40(4):1-8,26,9.

基金项目

山东省自然科学基金项目 ()

国家自然科学基金项目 ()

花生学报

1002-4093

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