湖南农业大学学报（自然科学版）2011，Vol.37Issue(6)：637-640,664,5.DOI:10.3724/SP.J.1238.2011.00637
1株烟草青枯病拮抗内生细菌的分离及鉴定
Isolation and identification of an antagonistic endophytic bacteria strain against tobacco bacterial wilt
摘要
Abstract
Tobacco bacterial wilt (TBW) is caused by bacterium Ralstonia solanacearum. To effectively develop microoganisms for biocontrol of TBW, 150 strains of tobacco endophytic bacteria from the stems of the health plant at TBW areas in Chenzhou, Yongzhou of Hunan were obtained by the dilution method. Antagonistic test showed 21 of the 150 strains, accounting for 14%, had antagonistic effects against R. Solanacarum. Among the 21 strains, strain F1 showed the best antagonistic effect with the diameter of inhibition zone against tobacco R. Solanacarum strain 2316 at 24 hours of 6.5 mm, and showed a wide antimicrobial spectrum including five kinds of pathogenic fungi such as Sclerotium rolfsii and five kinds of pathogenic bacteria such as Xanthomonas campestris pv. Citri. Based on analysis of the morphological, physiological, and biochemical characteristics and the 16S rDNA phylogeny, strain Fl was identified as Bacillus mojavensis.关键词
烟草/青枯雷尔氏菌/内生细菌/分离/鉴定Key words
tobacco/ Ralstonia solanacarum/ endophytic bacteria/ isolation/ identification分类
农业科技引用本文复制引用
周鑫钰,朱宏建,周倩,任佐华,吴凌婧,刘二明..1株烟草青枯病拮抗内生细菌的分离及鉴定[J].湖南农业大学学报（自然科学版）,2011,37(6):637-640,664,5.基金项目
国家自然科学基金项目(30900960) （30900960）
湖南省科学技术厅重大项目(2007NK2005) （2007NK2005）
湖南省烟草公司]项目(10-12Aa04) （10-12Aa04）
