湖南农业大学学报（自然科学版）2011，Vol.37Issue(6)：645-649,5.DOI:10.3724/SP.J.1238.2011.00645
信阳水牛PRL基因单核苷酸多态性与生长性状的相关性
Associations between single nucleotide polymorphisms in PRL gene and growth traits in Xinyang buffalo
摘要
Abstract
Blood samples from fifty xinyang buffalos were extracted for genomic DNA and the primers for PRL gene (NC-007324) were designed to detect the polymorphisms in the second, fourth and fifth exons of this gene by PCR-SSCP and DNA sequencing, then the correlations between the polymorphisms and the growth traits of the buffalos were analyzed. The PCR-SSCP and DNA sequencing showed that in the tested buffalos each exon had shifted SSCP band and there was one SNP in each exon (2974G>A, 7581G>A, 8558 G>-). The correlation analysis showed that the hucklebone widths in the buffalos genotyped AA were significantly (P<0.05) greater than those in the buffalos genotyped BB based on the second exon polymorphisms.关键词
信阳水牛/催乳素基因/单核苷酸多态性(SNPs)/生长性状Key words
Xinyang buffalo/ PRL gene/ single nucleotide polymorphisms (SNPs)/ growth traits分类
农业科技引用本文复制引用
马云,王伍,梁小娟,王璐,李芬,徐源,姚瑾..信阳水牛PRL基因单核苷酸多态性与生长性状的相关性[J].湖南农业大学学报（自然科学版）,2011,37(6):645-649,5.基金项目
河南省科技攻关计划项目(102102110106) （102102110106）
河南省高等学校青年骨干教师资助计划(85) （85）
