湖南农业大学学报（自然科学版）2012，Vol.38Issue(1)：73-77,5.DOI:10.3724/SP.J.1238.2012.00073
大鲵2个Sox基因HMG-box的克隆及分析
Cloning and sequence analysis of HMG-box of two Sox genes in Andrias davidianus
摘要
Abstract
Using a pair of degenerate primers designed based on HMG-box, a conserved region in Sox gene, two 217 bp-fragments were amplified and cloned from the male and the female Andrias davidianus which exhibited no sexual difference by PCR-SSCP analysis. Amino acid sequence analysis showed one of the 217 bp-fragments showed 90% homology to Sox 11 gene of human, zebrafish, mouse, chicken, Xenopus tropicalis and Alligator sinensis respectively and the other showed 90% homology to Soxl4 gene of Tilapia, Ornithorhynchus anatinus, mouse, Fugu rubripes, zebrafish, chicken, human and Xenopus Laeuis respectively, thereafter the two fragments were termed as adSoxl 1 and adSox14c respectively. Sox gene transcripts were found in spermary, ovary, heart and kidney by RT-PCR but not stomach and liver of Andrias davidianus. These results collectively suggested that Sox gene was highly conserved across species Sox gene and was selectively expressed among tissues and organs to accommodate the need of development.关键词
大鲵/Sox基因/高迁移率族蛋白盒Key words
Andrias davidianus/ Sox genes/ high mobility group box(HMG-box)分类
农业科技引用本文复制引用
许宝红,肖调义,肖真明,苏建明,刘巧林,陈开健,王冬武,徐永福..大鲵2个Sox基因HMG-box的克隆及分析[J].湖南农业大学学报（自然科学版）,2012,38(1):73-77,5.基金项目
国家自然科学基金项目(31040083) （31040083）
湖南省科技计划项目(2009NK3104) （2009NK3104）
