华西口腔医学杂志2011，Vol.29Issue(6)：571-575,5.DOI:10.3969/j.issn.1000-1182.2011.06.003
不同细菌密度的浮游牙龈卟啉单胞菌及其生物膜对甲硝唑敏感性的体外研究
Susceptibility of Porphyromonas gingivalis to metronidazole at different planktonic cell densities and in biofilm
摘要
Abstract
Objective To compare the susceptibility of Porphyromonas gingivalis to metronidazole at different plank-tonic cell densities and in biofilm, and to evaluate the role of cell density in antibiotic drug resistance in Porphyromonas gingivalis biofilm. Methods The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of metronidazole against Porphyromonas gingivalis were detected by a broth dilution method under a final inocula of 106CFU·mL-1 and 109CFU·mL-1 (cell number equal to biofilm). After the initial biofilm formed in the microtiter plate wells, the MIC and MBC of metronidazole to the intact and succedent resuspended biofilm were determined. Results The MIC and MBC of metronidazole against l06CFU·mL-1 planktonic Porphyromonas gingivalis were 0.063, 0.125 mg·L-1 respectively. However, those against l09CFU·mL-1 planktonic Porphyromonas gingivalis were 25, 50mg·L-1. Against intact Porphyromonas gingivalis biofilm, the MIC was 25mg·L-1 and MBC was higher than 125 mg·L-1, those against resuspended biofilm was 25, 125 mg·L-1 respectively. Conclusion The resistance of Porphyromonas gingivalis to metronidazole increases along with the augment of the bacterial density. Cell density plays an important role in the resistance of biofilm. However, extracellular matrix and the integrity of biofilm may be the other influence factors for the biofilm resistance.关键词
甲硝唑/牙龈卟啉单胞菌/生物膜/细菌密度Key words
metronidazole/Porphyromonas gingivalis/biofilm/cell density分类
医药卫生引用本文复制引用
段丁瑜,王爽,张利平,赵蕾,徐屹..不同细菌密度的浮游牙龈卟啉单胞菌及其生物膜对甲硝唑敏感性的体外研究[J].华西口腔医学杂志,2011,29(6):571-575,5.基金项目
国家自然科学基金资助项目(30872872,30801295) （30872872,30801295）
