华西口腔医学杂志2011，Vol.29Issue(6)：580-583,4.DOI:10.3969/j.issn.1000-1182.2011.06.005
表皮生长因子受体单抗MAb225对舌癌细胞DNA放射后损伤修复的影响
Influence of epidermal growth factor receptor monoclony antibody MAb225 on DNA repair of tongue carcinoma cell after radiation
摘要
Abstract
Objective The objective of this study is to investigate the effect of epidermal growth factor receptor (EGFR) monoclonal antibody MAb225 on repair of DNA double strand break (DNA-DSB) after radiation in tongue squamous cell carcinoma cell. Methods The single cell gel electrophoresis(SCGE) was performed to estimate the repair of DNA-DSB induced by radiation in human tongue carcinoma cells Tca8113 treated with or without MAb225. Expression of Ku70 and Ku80 were detected by semiquantitative reverse transcription polymerase chain reaction and Western bolt Results Comet tail moment of MAb225 treated cell was significantly higher than untreated cell(P＜0.05). The expression of Ku70 and Ku80 were inhibited by MAb225. Conclusion MAb225 can inhibit repair of DNA-DSB induced by down-regulated expression of Ku70 and Ku80.关键词
放射/舌癌/DNA/双链断裂/修复Key words
radiation/tongue carcinoma/DNA/double strand break/repair分类
医药卫生引用本文复制引用
王明国,王中和..表皮生长因子受体单抗MAb225对舌癌细胞DNA放射后损伤修复的影响[J].华西口腔医学杂志,2011,29(6):580-583,4.基金项目
山东省中青年科学家奖励基金资助项目(2006BS03046) （2006BS03046）
山东省十一五卫生科技发展计划基金资助项目(2007-11) （2007-11）
