华西口腔医学杂志2011，Vol.29Issue(6)：588-591,4.DOI:10.3969/j.issn.1000-1182.2011.06.007
碱性成纤维细胞生长因子对人牙周膜细胞多配体蛋白聚糖-4基因表达的影响
The effect of basic fibroblast growth factor on the gene expression of syndecan-4 by human periodontal ligament cell in culture
闫嘉晴 1林崇韬 1申玉芹 1刘彦 1张晓敏1
作者信息
- 1. 吉林大学口腔医院 牙周病科,长春130021
- 折叠
摘要
Abstract
Objective To study the effect of basic fibroblast growth factor (bFGF) on the gene expression of syn-decan-4 by human periodontal ligament cell (PDLC) in culture, and discuss the effect of bFGF on human PDLC proliferation and migration. Methods 68 adolescent (12-18 years old) health premolar were collected, which were extracted for orthodontic reason. Human PDLC were cultured and stimulated by exogenous bFGF. After cultured 24, 48, 72 h, gene expression of syndecan-4 was detected by SYBR green quantitative real time polymerase chain reaction. Results The mRNA expression of syndecan-4 in 24 h group increased markedly than that in control group (P＜0.01), expecially in l.0ng·mL-1 group. L.0ng·mL-1 group in 48 h higher than that control group (P＜0.05). 1.0 ng·mL-1 group in 72h compared with control group was lower (P＜0.05). Conclusion The mRNA expression of syndecan-4 was increased by bFGF at the beginning, but the expression was decreased with the time. The expression of such changes may be one of the important factors which participate in the migration process of PDLC.关键词
碱性成纤维细胞生长因子/牙周膜细胞/多配体蛋白聚糖-4.迁移Key words
basic fibroblast growth factor/periodontal ligament cell/syndecan-4/migration分类
生物科学引用本文复制引用
闫嘉晴,林崇韬,申玉芹,刘彦,张晓敏..碱性成纤维细胞生长因子对人牙周膜细胞多配体蛋白聚糖-4基因表达的影响[J].华西口腔医学杂志,2011,29(6):588-591,4.
