华西口腔医学杂志2011，Vol.29Issue(6)：640-642,3.DOI:10.3969/j.issn.1000-1182.2011.06.020
下牙槽神经缺失对下颌骨骨缺损骨痂血肿期炎症因子表达影响的实验研究
The effects of transection of inferior alveolar nerve on inflammatory factors during haematoma phases in mandibular defect healing of denervated rabbits
摘要
Abstract
Objective To Study the effects of transection of inferior alveolar nerve (IAN) on the healing of mandibu-lar fracture by investigating the changes of inflammatory factors expression, including tumor necrosis factor-α(TNF-α), interleukin-1 (IL-1) and interleukin-6 (IL-6) in the denervated rabbits' bony callus. Methods 50 Chinese rabbits were randomly divided into the experimental group and control group, the experimental group underwent mandibular fracture and transection of IAN and the control group underwent mandibular fracture only. The bony callus were collected at 1, 2, 3, 5 and 7 d after operation, respectively. The expression of TNF-a, IL-1 and IL-6 in callus was detected by enzyme linked immunosorbent assay (ELISA), and the mRNA of TNF-α was detected by reverse transcription-poly-merase chain reaction (RT-PCR). Results In the experimental group, at the 1st day afteT operation, the expression of TNF-α (75.1%), IL-1 (683%) and IL-6 (72.7%) was lower than that in control group (P＜0.05). At the 2nd day, the expression of IL-1 (75.5%) was still lower than that in control group, but the expression of TNF-α was higher than that in control group. In the next observation points, all inflammatory factors decreased, and there was no difference between experimental and control groups. In RT-PCR, the detection of TNF-α mRNA in the experimental group was lower than that in control group. Conclusion In the haematoma of mandibular fracture healing, transection of IAN causes neuro-. Humor decrease, depression and delay of inflammatory reaction. It is unfavorable to fracture healing.关键词
下颌骨骨缺损/下牙槽神经/肿瘤坏死因子-α/白细胞介素-1/白细胞介素-6Key words
mandibular defects inferior alveolar nerve/tumor necrosis factor-α/interleukin-1: interleukin-6分类
医药卫生引用本文复制引用
马高旗,李焰,张纲,谭颖徽..下牙槽神经缺失对下颌骨骨缺损骨痂血肿期炎症因子表达影响的实验研究[J].华西口腔医学杂志,2011,29(6):640-642,3.基金项目
国家自然科学基金资助项目(30973334,30772436) （30973334,30772436）
四川省卫生厅基金资助项目(100086) （100086）
