华西口腔医学杂志2012，Vol.30Issue(1)：25-28,31,5.DOI:10.3969/j.issn.1000-1182.2012.01.006
破骨细胞核因子κB受体活化因子配基和骨保护因子在种植体周围软组织及骨组织的表达
Expression of receptor activator nuclear factor kappa B ligand and osteoprotegerin in peri-implant soft tissue and bone tissue
摘要
Abstract
Objective To study mRNA expression of receptor activator nuclear factor kappa B ligand (RANKD and its decoy receptor, osteoprotegerin (OPG) in peri-implant tissue during unloading period. Methods An animal model of dental implant was established in 6 male Beagle dogs of 1-2 years old. Bone remodeling was tested at 3, 7, 15, 30, 60 and 90 days since the placement of implants. RANKL and OPG mRNA expression were quantified by real-time polymerase chain reaction (PCR). Then mandibular bones were taken out and the morphological changes were observed by X-ray, bone tissue was tested by immunohistochemistry stain. Results The most prominent period of bone remodeling occurred at 7th day after the placement of implants. The expression of RANKL and OPG increased in a timedependent manner in both soft and hard tissue. After 7 days they gradually decreased. Conclusion RANKL and OPG can express in soft tissue, and the changing tendency is consistent with the change of bone remodeling, it indicates that RANKL and OPG play an important role in the bone remodeling.关键词
牙种植/核因子κB受体活化因子配基/骨保护因子/种植体周Key words
dental implant/ receptor activator nuclear factor kappa B ligand/ osteoprotegerin/ peri-implant分类
生物科学引用本文复制引用
周文娟,柳忠豪,许胜,郝鹏杰,许丰伟,孙爱杰,卢志山..破骨细胞核因子κB受体活化因子配基和骨保护因子在种植体周围软组织及骨组织的表达[J].华西口腔医学杂志,2012,30(1):25-28,31,5.基金项目
山东省自然科学基金资助项目(Y2008C43) （Y2008C43）
