四川大学学报（医学版）2012，Vol.43Issue(1)：15-18,4.
人羊水干细胞分离方法及其生物学特性研究
Isolation and Biological Characterization of Human Amniotic Fluid-derived Stem Cells
摘要
Abstract
Objective To establish in vitro culture procedure for human amniotic fluid-derived CD117 positive stem cells, and to identify the characteristics of CD117 positive stem cells. Methods 86 amniotic fluid samples (10 mL of each) were obtained by second-trimester amniocentesis. Isolation of amniotic fluid-derived stem cells expressing CD117 antigen was performed via magnetic cell sorting using the CD117 MicroBead Kit. The karyotype of CD117 positive stem cells was analysed throughrepeated freezing. Adipogenic differentiation of these CD117 positive stem cells was displayed by Oil Red O staining. Osteogeneic differentiation of these CD117 positive stem cells was confirmed by Alizarin Red staining. Results The CD117 positive stem cells were successfully isolated and cultured from 61 samples, with all showing normal karyotype. Product analysis of specific staining confirmed that under specific culture mediums, these cells could be successfully induced to differentiate into adipocytes and osteocytes. Conclusion Based on this study, we estimate that isolating CD117 positive stem cells from second-trimester amniotic fluid obtained by amniocentesis has a success rate of 70. 93%. These cells maintain morphological and genetic stability in vitro. Human amniotic fluid-derived CD117 positive stem cells have the ability to differentiate in vitro into adipocytes and osteocytes under specific cuLture mediums and may be applied in cell transplantation and regenerative medicine.关键词
羊水/CD117/干细胞/分化Key words
Amniotic/fluid/CD117/Stem/cells/Differentiation引用本文复制引用
关婷,谢晓砚,刘珊玲,陈新莲,魏杨君,赖怡,谢良玉,刘之英,张雪梅,刘洪倩,张建军..人羊水干细胞分离方法及其生物学特性研究[J].四川大学学报（医学版）,2012,43(1):15-18,4.基金项目
国家自然科学基金(批准号30772323)和四川省科技厅科技支持计划项目(2008SZ0018)资助 （批准号30772323）
