四川大学学报（医学版）2012，Vol.43Issue(1)：19-23,5.
低氧性肺动脉高压大鼠肺内凝血酶敏感蛋白-1的表达
Expression of Thrombospondin-1 in the Lung of Hypoxic Pulmonary Hypertension Rats
摘要
Abstract
Objective To investigate the expression of thrombospondin-1 (TSP-1) in the lung of hypoxia-induced pulmonary hypertension rats. Methods Thirty male Wistar rats were divided into two groups, pulmonary hypertension group and control group. The mice in experimental group were exposed to isobaric hypoxia for 3 weeks, and those in control group were exposed to air. The pulmonary artery pressure was measured by right cardiac catheterization. The expression of TSP-1 and TGF-|31 in the lungs of rats were measured by immunohistochemical staining. The histological sections of the lungs were examined using a computerized image analyzer. Results After the induction of hypoxia for 3 weeks? The rats had pulmonary artery pressure increased with the thickening of the wall and the narrowing of the lumen of pulmonary arterioles. In the experimental group, the mean pulmonary artery pressure (mPAP) was (2. 86 + 0. 39) kPa, the index of right ventricular hypertrophy RV/(LV+S) was (43. 53 + 3. 38)%,the ratio of vascular wall thickness/vascular external diameter (WA%) was (55.09±12. 38)%, and the ratio of vascular wall area/total vascular area (WT%) was (35. 24±11.2)%, which all were significantly increased in comparison with those of control group [mPAP (1. 35±0. 28) kPa, RV/(LV + S) (23. 68±3. 48)%, WT% (23. 63±9. 74)% , WA% (41. 62± 12. 83)%, respectively. P<0. 053. The positive staining of TSP-1 (1. 32 ± 0. 04 vs. 0. 96 ± 0. 03) and TGF-01 (1.38 ± 0.05 vs. 1. 04 ± 0. 04) in the wall of pulmonary arteriole of the rats exposed to hypoxia were significantly stronger than those of control rats(P<0. 01). Conclusion The expression of TSP-1 appears to be increased in hypoxic pulmonary hypertension rats, which may contribute to the pathogenesis of hypoxic pulmonary vascular remodeling.关键词
低氧/肺动脉高压/肺血管重建/凝血酶敏感蛋白-1Key words
Hypoxia/Pulmonary/hypertension/Pulmonary/vascular/remolding Thrombospondin-1引用本文复制引用
杨艳娟,程德云,郑西卫,李芳,杨桂兰..低氧性肺动脉高压大鼠肺内凝血酶敏感蛋白-1的表达[J].四川大学学报（医学版）,2012,43(1):19-23,5.基金项目
国家自然科学基金(批准号30960142)资助 （批准号30960142）
