四川大学学报（医学版）2012，Vol.43Issue(1)：50-53,59,5.
一株水源分离军团菌的分子生物学鉴定和系统发育学及毒力分析
Molecular Identification, Phylogenic and Virulence Analysis of a Legionella Strain Isolated from Aquatic Environment
摘要
Abstract
Objective CD-1 was isolated from aquatic environment, which grew with strict L-cysteine dependence. In this study, we applied molecular methods to identify CD-1, and animal test to understand its virulence. Methods To identify CD-1 strain, CD-1 strain was tested for genus-specific 16S rRNA of Legionella via PCR amplification, then its rpoB gene was sequenced for phylogenic analysis. To understand the virulence, CD-1 was detected for mip gene, which was an indispensable virulent gene of Legionella. Then, BABL/c mice were infected by CD-1 in different dosages. Results For identification, CD-1 was positive for genus-specific 16S rRNA of Legionella, while in the phylogenic tree CD-1 was a sister to Legionella longbeachae with high posterior probability (PP=1. 00). For the virulence analysis, CD-1 was positive for mip gene detection. In the animal test, all mice tested died when the infection dose of CD-1 strain reached 107 cfu/mL. Conclusion CD-1 strain was identified to be Legionella longbeachae with strong virulence to BALB/c mice. It may be a potential virulent strain to human. This is the first strain of Legionella longbeachae isolated in Sichuan province, and this is the first virulence analysis of Legionella strain isolated from aquatic environment in China.关键词
军团菌/水环境/系统发育学/毒力Key words
Legionella/Aquatic/environment/Phylogenic/analysis/Virulence引用本文复制引用
关望,陈建平,徐佳楠,许颖..一株水源分离军团菌的分子生物学鉴定和系统发育学及毒力分析[J].四川大学学报（医学版）,2012,43(1):50-53,59,5.基金项目
国家自然科学基金(批准号81001345,30771883)和教育部博士学科点专项科研基金(20060610091)资助 （批准号81001345,30771883）
