海洋科学2011，Vol.35Issue(8)：51-57,7.
日本蟳血细胞及血液生化指标的研究
Hemocytes and blood biochemical characterization of Charybdis japonica
摘要
Abstract
In order to provide data for tissue pathology, disease control, and reproductive physiological research of Charybdis japonica, this paper reported the hemocytes and the blood biochemical characterization of Charybdis japonica. With light and electronic microscopes, four types of hemocytes, including agranular hemocyte （AG）, small granular hemocyte （SG）, intermediate granular hemocyte （IG） and large granular hemocyte （LG）, were classified according to their granular existence, granular size：, refractivity, staining characters, and nuclear-cytoplasmic ratio. The nuclear-cytoplasmic ratios were 53.23%（AG）, 33.64%（SG）, 24.50% （IG） and 19.45% （LG）, respectively. Among these hemocytes, the percentages of AG, SG, IG and LG were 4.78%, 42.69%, 36.57%, and 15.96%, respectively. The female crab had higher density of hemocytes than the male, but there was no significant difference in cell size. The phagocytic capacity of hemocytes from strong to weak were IG, LG and SG. AG had no phagocytic capacity. The hemocytes in gill had big pseudopodia and abundant residual body suggesting they could be more active in phagorytosis. In the developing phase, the values of total protein（TP）, glucose（GLU） and cholesterin（CHOL） of female were higher than those of male, but the male crabs in maturation had more TP than the female. After spawning, the female crabs could maintain high levels of TP and CHOL. After starvation for seven days, the values of density of hemocytes, TP, GLU, and CHOL decreased obviously and there were lower activities of alanine aminotransferase（ALT） and alkaline phosphatase（ALP） than before, but the levels of creatinine（CREA） and usea nitrogen（BUN） increased. Globin was found in the blood serum of Charybdis japonica.关键词
日本蟳（Charybdis/japonica）/血细胞/血液生化指标/吞噬能力/饥饿Key words
Charybdis japonica/hemocyte/blood biochemical value/phagocytic capacity/starvation分类
生物科学引用本文复制引用
许星鸿,阎斌伦,郑家声,邵营泽,徐加涛,徐国成,周盟,周黎..日本蟳血细胞及血液生化指标的研究[J].海洋科学,2011,35(8):51-57,7.基金项目
基金项目：江苏省海洋生物技术重点建设实验室开放课题 （）
江苏省科技厅苏北科技发展计划项目 （）
