海洋科学2011，Vol.35Issue(10)：57-63,7.
温度和盐度对马氏珠母贝精子激活率及活力的影响
Effect of temperature and salinity on the sperm activation and motility in Pinctada fucata martensii （Dunker）
摘要
Abstract
Central composite circumscribed experimental design and response surface analysis method was employed to study effects of different combinations of temperature and salinity on the sperm activation and relative motility of Pinctada fucata martensii （Dunker）, and the equation of temperature, salinity and sperm activation rate and motility was established. The temperature was raning from 15 to 45℃, and the salinity 12 to 48. The sperm activation rate and motility were first increased and then decreased as temperature and salinity increased; The sperm activation rate and motility were significantly affected by the linear and quadratic effects of salinity and quadratic effects of temperature （P〈0.01）, but The synergistic effect between salinity and temperature was not significant （P〉0.05）; the suitable temperature and salinity of sperm activation rate were 26-31℃ and 25-30, respectively, and of sperm motility were 24-35℃ and 22-30, respectively. By means of optimization technique, the optimal point was found at temperature 30.04℃ and salinity 26.86. At this optimum point, maximumal sperm activation rate and sperm motility were 85.51% and 57.82%, respectively, with the desirability as high as 97%.关键词
马氏珠母贝（Pinctada/martensii）/精子激活/活力/响应曲面/优化Key words
Pinctadafucata martensii/sperm activation/sperm motility/response surface/optimization分类
生物科学引用本文复制引用
王辉,罗明明,朱晓闻,吕文刚,李艳红,王亚男,刘志刚..温度和盐度对马氏珠母贝精子激活率及活力的影响[J].海洋科学,2011,35(10):57-63,7.基金项目
基金项目：广东省高校热带海产无脊椎动物养殖工程研究中心科研项目 （）
国家科技支撑计划项目 （）
农业部公益性行业（农业）科研专项子课题 （）
广东省重大科技兴海（兴渔）项目 （）
