海洋科学2011，Vol.35Issue(12)：82-87,6.
大黄鱼野生群体与养殖群体遗传多样性研究
Study of genetic diversity of wild and culture populations of Pseudosciaena crocea using two molecular markers
摘要
Abstract
Large yellow croaker, Pseudosciaena crocea Richardson 1846, is a commercially important marine fish and now is very scarce. The fish could be found only occasionally in the Lvsu Fisheries Region in Jiangsu（JS） province, and no researches were carried out about this wild population. In the present study, partial mitochondrial cytochrome oxidase I gene （COI） gene sequence and inter-simple sequence repeat （ISSR） markers were used to investigate the genetic diversity of this wild population and two cultivated populations from Zhejiang（ZJ） and Fujian（FJ） province. The results were as follows： （1） The amplified fragment was 625 bp, revealing 15 haplotypes and three haplotypes of which were shared by these populations. The JS wild population has nine unique haplotypes and two haplotypes shared with the ZJ population. Nei＇s coefficient of differentiation（Gst） between populations were -0.0196 （JS - ZJ）, 0.02115（JS- FJ） and -0.0125（FJ-ZJ）, respectively; （2） Ten informative and reliable primers were selected to investigate the genetic diversity. The proportation of polymorphic loci of JS, ZJ and FJ populations were 74.59%, 63.93% and 59.02%, respectively. Nei＇s genetic identity between populations were 0.9246（JS-ZJ）, 0.8310（JS-FJ） and 0.8826（FJ-ZJ）, respectively.关键词
大黄鱼（Pseudosciaena/crocea）/COI/ISSR/遗传多样性/群体遗传差异Key words
Pseudosciaena crocea COI partial sequence ISSR genetic diversity population genetic differentiation分类
生物科学引用本文复制引用
陈淑吟,徐士霞,张志勇,郭忠仁,孙中响,朱立静..大黄鱼野生群体与养殖群体遗传多样性研究[J].海洋科学,2011,35(12):82-87,6.基金项目
“十一五”国家科技支撑计划重大项目 （）
