海洋科学2012,Vol.36Issue(1):10-16,7.
许氏平鲉4个野生群体遗传多样性微卫星分析
Microsatellite analysis of genetic diversity in four wild stocks of black rockfish Sebastes schlegeli
摘要
Abstract
The genetic diversity of four wild stocks of black rockfish Sebastes schlegeli including Rongcheng stock 1, Rongcheng stock 2, Jimo stock and Longkou stock, was investigated using 18 microsatellite DNA loci. The results showed that the mean effective numbers of alleles(Ne) were 3.1, 2.7, 2.8 and 2.7, respectively. The values of mean expected heterozygosity(He) were 0.590, 0.540, 0.552 and 0.491, respectively. And the values of mean poly- morphism information content(P/C) were 0.577, 0.495, 0.538 and 0.528, respectively. These four stocks had abun- dant genetic diversity, but most loci were also found to be significantly deviated from HWE in which heterozygote absence was apparent according to the results of chi-square test (P〈0.05). Genetic differentiation and hierarchical partition of genetic diversity were evaluated by Fst, which indicated high values of variation within four stocks and low levels of genetic variation among stocks (Fst=0.04). The results would provide basic data for the conservation and utilization of natural S.schlegeli breeds, and for its artificial releasing.关键词
许氏平鲉(Sebastes/schlegeli)/野生群体/微卫星标记/遗传多样性Key words
Sebastes schlegeli/wild stock/microsatellite DNA/genetic variation分类
生物科学引用本文复制引用
王文琪,张毅,刘梦侠,吴志昊,王丽娟,訾珺,关健,尤锋..许氏平鲉4个野生群体遗传多样性微卫星分析[J].海洋科学,2012,36(1):10-16,7.基金项目
基金项目:国家海洋公益性行业科研专项 ()