渔业科学进展2011，Vol.32Issue(6)：43-49,7.
泥鳅血清凝集素的研究
Preliminary study on lectins from serum of loach Misgurnus anguillicaudatus
摘要
Abstract
Two lectins with different molecular mass, designated as MSL-I and MSL-Ⅱ, were isolated from serum of loach Misgurnus anguillicaudatus. The purification procedure consisted of separation on DEAE-Sepharose FF anion exchange column followed by gel filtration chromatography on Superdex 200 10/300 GL. Determination showed that MSL-I and MSL-II had a nature molecular weight of 25,000 and 82,000 respectively. The denatured SDS-PAGE showed that both MSL-I and MSL-II had two bands on the gel. We proposed that MSL-Ⅱ was constituted with two subunits with molecular mass of 29,000 and 13,600 respectively. The sub-unit composition of MSL-I was to be further studied. Sugar-binding specificity test showed that glucose, D-galactose, D-fructose, D-xylose, L-arabinose and lactose had inhibitory effect on MSL-Ⅱ, and among them lactose was most effective. Thermal stability test showed that MSL-II had a strong thermal stability even treated at 60°C for 30min. The optimum pH of MSL-Ⅱ was 6~8.关键词
泥鳅/血清/凝集素/分离纯化Key words
Loach Misgurnus anguillicaudatus Serum Lectin Purification分类
生物科学引用本文复制引用
姚东瑞,盘赛昆,周鸣谦,葛荣荣..泥鳅血清凝集素的研究[J].渔业科学进展,2011,32(6):43-49,7.基金项目
江苏省科技支撑计划(农业)项目(BE2010399)、江苏省农业科技自主创新基金项目[CX09-627 （农业）
CX(10)328]和连云港市科技基础设施建设计划项目(CK0935)共同资助 （10）
