华中师范大学学报（自然科学版）2011，Vol.45Issue(4)：595-598,4.
酒石酸长春质碱与DNA相互作用的光谱研究
Study on the interaction between catharanthine tartrate and DNA by spectrometric method
摘要
Abstract
The interaction of catharanthine tartrate and DNA was studied using UV spec-trometry, fluorescence spectrometry and fluorescent probe. The results showed that the fluorescence intensity of catharanthine tartrate was quenched in the presence of DNA, and this mechanism was judged as static quenching. The binding constant K at 25℃ and 40℃ were calculated as 5. 38 × 104 and 5. 85 × 102, and the binding sites number n as 1. 405 and 0. 8306 . According to the binding constant K at 25℃ and 40℃ ,the thermo-dynamic parameters of the reaction of catharanthine tartrate and DNA were calculated as follows: △Hm= -233. 77 kJ ? Mol-1, △Gm(298 K) = -27. 0 kJ ? Mol-1, △Sm(298 K) = -693. 8 J ? K-1 ? Mol-1, and the main binding force between catharanthine tartrate and DNA is hydrogen bonding or van der Waals force according to thermodynamic parameters. The UV spectrometry test showed that as the DNA concentration increased, the blue shift of the absorbance peak occurred and the absorbance decreased significantly, the results of fluorescence probe test further proved that catharanthine tartrate binds with DNA in a mode of groove binding.关键词
酒石酸长春质碱/DNA/相互作用/光谱Key words
catharanthine tartrate/ DNA/ interaction/ spectrometry分类
化学化工引用本文复制引用
刘炜,张琼梅,史载锋,何文英..酒石酸长春质碱与DNA相互作用的光谱研究[J].华中师范大学学报（自然科学版）,2011,45(4):595-598,4.基金项目
海南省教育厅项目(Hjkj2009-39) （Hjkj2009-39）
海南省自然科学基金项目(209004). （209004）
