家畜生态学报2011，Vol.32Issue(6)：16-20,5.
MyoG和A-FABP基因多态性与鹅肉品质性状的相关性研究
Relativity Study on the Polymorphism of Intron 2 of A-FABP and Exon 1 of MyoG Gene with the Meat Quality in Goose
摘要
Abstract
In order to detect the relativity of single nucleotide polymorphism of MyoG gene and adipo-cyte fatty acid binding protein (A-FABP) gene with meat quality in the geese, the crude fat and some muscle fiber traits were detected, using the techniques of PCR-SSCP and sequencing to study the single nucleotide polymorphism of exon 1 of MyoG gene and intron 2 of A-FABP of Taihu geese, and then relativity a-nalysis was conducted. The results showed; (1) The PCR products of exon 1 of MyoG gene had about 420 bp, the mutational site was on the 64 bp (C→T). There was a significant difference of muscle fiber density and muscle fiber diameter between different genotypes(P<0. 05). (2) The products of PCR had about 482 bp, the intron 2 of A-FABP was from 174 bp to 394 bp, there were three mutational sites, respectively on the 187 bp (C→T), 235 bp (C→A) and 372 bp(A→C); (3) By using PCR-SSCP ,two genotypes of the intron 2 of A-FABP were found, there had some difference between different genotypes. Compared the sequence of intron 2 of goose with duck and chicken, the homology were 91% and 85%, respectively. These findings laid good theoretical foundation for the progressive research of the relationship between genetic mutation in MyoG and A-FABP gene with meat trait and for the progressive research of the application of molecular markers in animal breeding.关键词
鹅/MyoG基因/A-FABP基因/单核苷酸多态性Key words
goose/ MyoG gene/ A-FABP gene/ single nucleotide polymorphism分类
农业科技引用本文复制引用
詹修君,高玉时,陆俊贤,张晶鑫,张小燕,唐梦君..MyoG和A-FABP基因多态性与鹅肉品质性状的相关性研究[J].家畜生态学报,2011,32(6):16-20,5.基金项目
国家科技支撑计划(2008BADB2B08) （2008BADB2B08）
