家畜生态学报2011，Vol.32Issue(6)：27-32,6.
德宏奶水牛DGAT1基因多态性及其与产奶性状的相关性分析
The Polymorphisms of DGAT1 and Its Association with Milk Production Traits in Dehong Milk Buffalo
摘要
Abstract
To reveal the SNPs of DGAT1 gene and evaluate its relationship with milk production traits in Dehong milk buffalo (Murrah× Dehong). 81 Dehong milk buffalo were used to detect the SNPs of DGAT1 gene exon 8 and intron 8 by PCR-SSCP and sequencing technology, and then analyzed its relationship with milk yield, fat percentage and protein percentage by general linear model (GLM). Three genotypes CT-GG, CCGG and CCGT were explored in DGAT1 gene. Sequencing result displayed that two mutations were detected which were 14 C→T and 35 G→T in intron 8, the K232A locus was not detected in Dehong milk buffalo population. Correlation analysis showed that animals with the CCGT genotype was significant in fat percentage than those with genotypes CTGG and CCGG (P＜0. 05).关键词
DGAT1基因/SNPs/产奶性状/分子标记/水牛Key words
DGATI gene/ SNPs/ milk production traits/ molecular marker/ buffalo分类
农业科技引用本文复制引用
樊月圆,王强,张永云,杨忠,富国文,汤守锟,田建云,李卫真..德宏奶水牛DGAT1基因多态性及其与产奶性状的相关性分析[J].家畜生态学报,2011,32(6):27-32,6.基金项目
云南省应用基础研究面上项目(2007C057M) （2007C057M）
