家畜生态学报2011，Vol.32Issue(6)：57-60,4.
鸡传染性贫血病毒病LAMP快速可视化检测方法的建立
Development of a Reverse Transcription Loop-mediated Isothermal Amplification Assay for Visual Detection of Chicken Infectious Anemia
摘要
Abstract
A loop-mediated isothermal amplification (LAMP) assay was developed for detection of chicken infectious anemia(CIAV). According to the sequences of CIAV in GenBank, six primers were designed, and the reaction conditions were optimized. The results showed that the detection limit of this LAMP method was 10 fg, which was higher than the routine PCR. The amplification could be finished within 1h, and the presence of CIAV could be detected by naked eyes. There was no cross-reactivity with the other pathogen of chiken. These results suggest that this LAMP assay is a simple and specific method for rapid detection of CIAV in clinical samples.关键词
鸡传染性贫血病毒病/LAMP/检测Key words
chicken infectious anemia(CIAV)/ LAMP/ detection分类
农业科技引用本文复制引用
邓显文,谢芝勋,谢志勤,刘加波,庞耀珊,谢丽基,彭宜,范晴..鸡传染性贫血病毒病LAMP快速可视化检测方法的建立[J].家畜生态学报,2011,32(6):57-60,4.基金项目
广西科技攻关项目(桂科攻0537008-3A （）
桂科攻1123007-4) （）
广西特聘专家专项经费(2011B020) （2011B020）
新世纪百千万人才工程国家级人选专项经费(945200603) （945200603）
