家畜生态学报2011，Vol.32Issue(6)：71-73,3.
牛乳铁蛋白卵黄抗体的制备及纯化方法的建立
The Establishment of Preparation and Purification Method of Egg Yolk Antibody of Bovine Lactoferrin
江青东 1王慧杰1
作者信息
- 1. 郑州牧业工程高等专科学校继续教育学院,河南郑州450011
- 折叠
摘要
Abstract
Through self-preparing and purifying the egg yolk antibody of bovine lactoferrin, the poly-clonal antibody was prepared by combining the methods of muscle and subcutaneous to immune the Roman laying hens. Four Roman laying hens at 150d ages were selected to conduct the subcutaneous immunization several times with different densities. The egg yolk antibodies were collected and purified preliminarily with the method of ammonium sulfate precipitation. After four times of immunization, the egg yolk antibody titer of Roman laying hens arrived at 1 : 32 000 and 1 : 24 000 and lasted for over two weeks at high levels, and the purity of the antibody after preliminary purification improved greatly. The results show that it is feasible to prepare polyclonal antibody through the method of combination of muscle and subcutaneous immunization and it can meet the needs of experiment with the method of ammonium sulfate precipitation.关键词
牛乳铁蛋白/卵黄抗体/制备/纯化Key words
bovine lactoferrin/ egg yolk antibody/ preparation/ purification分类
农业科技引用本文复制引用
江青东,王慧杰..牛乳铁蛋白卵黄抗体的制备及纯化方法的建立[J].家畜生态学报,2011,32(6):71-73,3.
