基础医学与临床2012，Vol.32Issue(1)：12-15,4.
小鼠间充质干细胞脂向分化过程中miRNA-143的表达
The expression of miRNA-143 during adipogenic differentiation of mouse MSCs
摘要
Abstract
Objective To investigate the expression of miR-143 during adipogenic differentiation of mesechymal stem cells(MSCs)in mice, and provide new clues to clarify the regulation mechanisms of MSCs' adipogenic differentiation. Methods The MSCs of C57BL/6 mice were isolated, cultured by using the whole bone marrow method, amplified by the differential adherent method, and adipogenic differentiation of the fifth generation of MSCs was induced with the adipogenic medium. We used the microRNA microarray to explore the expression of miR-143 in the MSCs group and adipocytes group, and validated it by real-time quantitative polymerase chain reaction (real-time PCR). Results We got high-purity MSCs and Oil 0 staining showed that lipid droplets were increased in MSCs treated with adipogenic conditions. The miR-143 was up-regulated during adipogenic differentiation showed by mi-croRNA microarray analysis and confirmed by using real-time PCR(3.73 ±0. 42 us 1. 00 ±0. 14,P<0.01). Conclusions MiRNA-143 may regulatie the adipogenic differentiation of MSCs.关键词
miRNA-143/间充质干细胞/脂向分化/miRNA芯片/实时定量PCRKey words
miR-143/mesenchymal stem cells/adipogenic differentiation/microRNA microarray/real-time PCR分类
医药卫生引用本文复制引用
凌宏艳,胡小波,奉水东,杨丝丝,何剑琴,张恺芳,廖端芳..小鼠间充质干细胞脂向分化过程中miRNA-143的表达[J].基础医学与临床,2012,32(1):12-15,4.基金项目
国家自然科学基金(81000328,30971170) （81000328,30971170）
南华大学博士科研启动基金(2010XQD39) （2010XQD39）
