基础医学与临床2012，Vol.32Issue(1)：16-20,5.
PINK1参与调节多巴胺的合成
The regulatory effects of PINK1 on dopamine biosynthesis
摘要
Abstract
Objective To explore the role for PINK1 in the regulation of dopamine biosynthesis. Methods The dopaminergic MN9D cells were used as a cell model for the present study. P1NK1 gene was knockdown by RNAi technique. The dopamine (DA) level, mRNA and protein expression level of tyrosine hydroxylase (TH) and dopa decarboxylase ( DDC) were then detected by HPLC with electrochemical detection, real-time RT-PCR or Western blots 48h later, respectively. The association between PINK1 and TH was observed with Co-IP and immunohisto-chemistry. Results When PINK1 was silenced, DA content was 5. 356 ± 0. 536 |μg/L significantly lower than the control group of 11. 630 ± 1. 559 μg/L ( P < 0. 05 ) , TH mRNA and protein expression were 0. 371 ± 0. 140 and 0. 195 ±0. 062 significantly lower than that of control group 1. 009 ±0. 152 and 0. 386 ±0. 044 (P <0.05), DDC mRNA and protein expression were 0. 396 ±0. 124 and 0. 149 ±0. 029 significantly lower than that of control group1. 009 ±0. 152 and 0. 386 ±0. 044, respectively (P <0. 05). Conclusions PINK1 has a regulatory effect on do-pamine biosynthesis, through its influence on TH and DDC.关键词
PINK1/RNA干扰/酪氨酸羟化酶/MN9D细胞Key words
PINK]/RNA interference/tyrosine hydroxylase/MN9D cells分类
医药卫生引用本文复制引用
贾焕珍,鲁玲玲,龚普盛,付越姣,赵春礼,段春礼,杨慧..PINK1参与调节多巴胺的合成[J].基础医学与临床,2012,32(1):16-20,5.基金项目
国家重点基础研究发展计划(2011CB504103) （2011CB504103）
国家自然科学基金(30970940) （30970940）
北京市教育委员会科技发展计划重点项目(KZ201010025022) （KZ201010025022）
北京市自然基金面上项目(5102012,5102007) （5102012,5102007）
北京市高校人才强教资助PHR(200907113) （200907113）
