基础医学与临床2012，Vol.32Issue(1)：36-41,6.
吡咯烷二硫代氨基甲酸酯减轻2型糖尿病大鼠胰岛β细胞氧化损伤
Pyrrolidine dithiocarbamate attenuates pancreatic β-cell oxidative injury in type 2 diabetic rats
摘要
Abstract
Objective To investigate the effect and mechanism of pyrrolidine dithiocarbamate (PDTC) on islet p cell oxidative injury in type 2 diabetic rats. Methods Type 2 diabetic rat model was set up by long-term feeding high-fat foods accompanied with a single intraperitoneal injection of low dose of streptozotocin ( STZ, 27 mg/kg body weight). The rats in PDTC group were administerd with PDTC (50 mg/kg ? D) by intraperitoneal injection. After treatment for 1 week, blood glucose levels were estimated. Malondialdehyde (MDA) , Superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) content in pancreatic tissue was measured. The expression of induc ible nitric oxide synthase (iNOS) and the prouduction of nitrotyrosine (NT) in islet tissue were examined byusing immunohistochemistry and Western blot. The rate of pancreatic islet β cell apoptosis was detected by flow cy-tometry. Results The blood glucose level, MDA content, pancreatic p-cell apoptosis, iNOS expression and NT production were significantly higher, while the SOD and GSH-PX activities were significantly lower in DM group than those in normal control group ( P < 0. 01). After administration of PDTC, The level of blood glucose, MDA content, pancreatic p-cell apoptosis, iNOS expression and NT production were significantly decreased, while the SOD and GSH-PX activities were markedly increased when compared with DM group ( P < 0. 01 ). Conclusions PDTC can relieve the oxidative stress reaction of diabetic rats, suppress the expression of iNOS and NT production, and decrease pancreatic islet β cell apoptosis in diabetic rats.关键词
PDTC/糖尿病/氧化损伤/胰岛β细胞/凋亡Key words
pyrrolidine dithiocarbamate/diabetes/oxidative injury/pancreatic islet β cell/apoptosis分类
医药卫生引用本文复制引用
丁海燕,赵瑞景,尹小妹,刘桂红,刘建坤,朱铁年..吡咯烷二硫代氨基甲酸酯减轻2型糖尿病大鼠胰岛β细胞氧化损伤[J].基础医学与临床,2012,32(1):36-41,6.基金项目
河北省自然科学基金(C2009001232) （C2009001232）
河北省科技支撑计划项目(10276105D-66) （10276105D-66）
河北省卫生厅资助项目(20090168) （20090168）
国家人事部留学人员科技活动项目择优资助项目(2008) （2008）
