基础医学与临床2012，Vol.32Issue(1)：61-65,5.
抗TNF-α抗体治疗大鼠肝肺综合征
The treatment of hepatopulmonary syndrome using anti-tumor necrosis factor-α monoclonal antibody in rats
摘要
Abstract
Objective To study the effects of anti-tumor necrosis factor-α monoclonal antibody on the expression of NF-kB on hepatopulmonary syndrome in rats and the effects of NF-kB in treating hepatopulmonary syndrome in rats with anti-tumor necrosis factor-a monoclonal antibody. Methods The experimental models of HPS in rats were developed with commom bile duct ligation, and then were injected with anti-TNF-aMcAb(0. 1 mg/kg ? 2 d)intraper-itoneally, Lung histopathological changes were evaluated by hematoxylin and eosin staining; TNF-ot concentration in plasma was measured by radioimmunity method; The endotoxin in the plasma was checked by tachypleus amebo-cyte lysate kit; Hepatopulmonary syndrome was assessed by measurements of alveoloarterial oxygen difference (P( A-a)O2). Western blot was employed to investigate the expression and the changes of NF-kB in hepatopulmonary rat lungs. Results Compared with the CBDL group, there was a gentle vasodilatation and widen interalveolar septum in lung in anti-TNF-αMcAb treament group, the level of the P(A-a)O2 was significantly lower (P <0. 05).The concentration of ETX and TNF-a were significantly lower(P <0. 05). The expression of NF-kB in CBDL + an-ti-TNF-α McAb group (0. 75 ±0. 18) decreased obviously than those in CBDL group (0. 93 ±0. 23) (P <0. 05). Conclusions Anti-tumor necrosis factor-α antibody can reduce the expression of NF-kB and attenuate the severity of hepatopulmonary syndrome through downregulateing the expressiion of NF-kB.关键词
肿瘤坏死因子-α/肿瘤坏死因子-α单克隆抗体/肝肺综合征/核转录因子-κBKey words
TNF-α/anti-TNF-αMcAb/hepatopulmonary syndrome/NF-kB分类
医药卫生引用本文复制引用
冯英梅,刘丽,刘楠,赵芝..抗TNF-α抗体治疗大鼠肝肺综合征[J].基础医学与临床,2012,32(1):61-65,5.基金项目
河北省医学科学研究重点课题(08080) （08080）
