基础医学与临床2012，Vol.32Issue(2)：133-137,5.
骨髓Sca-1+间充质干细胞移植减轻小鼠迟发型超敏反应
Sca-1 + bone marrow mesenchymal stem cells transplantation for the alleviation of delayed type hypersensitivity in mouse
曲学彬 1曾洋 1刘星霞 1赵春华1
作者信息
- 1. 中国医学科学院北京协和医学院基础医学研究所组织工程中心,北京100005
- 折叠
摘要
Abstract
Objective To observe the immunoregulatory effects of Sca-1 + bone marrow mesenchymal stem cells (BMSCs) on the delayed type hypersensitivity (DTH) mouse. Methods BALB/c mice were randomly divided into two groups, control group and MSCs infusion group. MSCs infusion group mice were intraperitoneally injected with Sca-1 + BMSCs from Balb/c mice on 0, 2 and 6 d, while control group mice were injected with normal saline. All the recipient mice were then injected with C57BL/6 splenocytes into the dorsal flank on 7 and 14 d into the hind footpad. After 24 h, footpad thickness, cytokines levels in the peripheral blood and the proportion of regulatory immune cells in spleen were measured by micrometer, ELJSA and FACS, respectively. Results Footpad thick-ness of MSCs infusion group mice [ (0. 368 ±0. 126)mm] was significantly lower than that in the control group [(0.731 ±0.1ll)mm, P <0. 01]. In the peripheral blood of MSCs infusion group mice, levels of IL-10 and TGF-B. Were higher than that of control group mice; while levels of IL-12 and TNF-α were lower than that of control group (P < 0. 05). The proportion of regulatory T cells and dendritic cells in the MSCs infusion group mice exceeded that of control group mice (P < 0. 05). Conclusions Transplantation of BMSCs alleviated DTH in vivo via up-regulating the levels of anti-inflammatory cytokines and regulatory immunocytes.关键词
间充质干细胞/迟发型超敏反应/免疫反应Key words
mesenchymal stem cells/delayed type hypersensitivity/immune response分类
医药卫生引用本文复制引用
曲学彬,曾洋,刘星霞,赵春华..骨髓Sca-1+间充质干细胞移植减轻小鼠迟发型超敏反应[J].基础医学与临床,2012,32(2):133-137,5.
