基础医学与临床2012，Vol.32Issue(2)：138-142,5.
Slug减轻照射鼠肠上皮细胞损伤及作用机制
The protection effect and mechanism of Slug on irradiation of rat intestinal epithelial cells
迟绍云 1焦学龙 2王宝泉 1刘春生 1王蕾 1陈栋2
作者信息
- 1. 青岛大学医学院 附属海阳市人民医院普外科,山东海阳265100
- 2. 青岛大学医学院 附属医院普外科,山东青岛266003
- 折叠
摘要
Abstract
Objective To investigate the protection effect and mechanism of slug overexpression on irradiation of rat intestinal epithelial cells. Methods The intestinal crypt epithelial cells (IEC6) were used and transfected with Slug cDNA ire vitro. The cells were irradiated with 6 Gy 60Co gamma rays. 48 hours after the irradiation, apoptosis indexes were calculated. The expression of PUMA was observed by the immunohistochemistry and Western blot.Results The apoptosis indexes in IEC6 by the irradiation of 60Co gamma rays after 48 hours were significantly higher than that in pcDNA3-Slug cDNA and in pcDNA3-Slug cDNA without irradiation (15. 60 ± 1. 54 versus 5. 10 ± 1. 70; 2. 21 ±0. 36, P <0. 01). The expression of PUMA in IEC6 by the irradiation of 60Co gamma rays after 48 hours was significantly higher than that in pcDNA3-Slug cDNA by Western-blot (0. 79 ±0. 12 versus 0. 16 ±0. 01, P <0. 01). The immunohistochemistry and Western blot assay results were consistent. Conclusions Upregulation of Slug can restrain the upregulation expression of PUMA in irradiated cells and the apoptosis of IEC6.关键词
Slug/PUMA/射线照射/凋亡/鼠肠上皮细胞Key words
Slug/PUMA/ray irradiation/apoptosis/rat intestinal epithelial cells分类
医药卫生引用本文复制引用
迟绍云,焦学龙,王宝泉,刘春生,王蕾,陈栋..Slug减轻照射鼠肠上皮细胞损伤及作用机制[J].基础医学与临床,2012,32(2):138-142,5.
