基础医学与临床2012，Vol.32Issue(3)：251-256,6.
过表达突变的NPM1基因对THP-1白血病细胞增殖和凋亡的影响
Effects of overexpression nucleophosmin (NPM1) mutations on the proliferation and apoptosis of THP-1 leukemic cells
摘要
Abstract
Objective To investigate the effect of Nucleophosmin ( NPM1) mutations on the proliferation and apop-tosis of leukemia cells. Methods The pEGFPC1-NPM1 mA plasmid vector was transfected into THP-1 cells to establish the stably expressed NPM1 mutation A protein leukemia cells (THP-1 mA). The cells transfected with pEGFPCl plasmid (THP-1 C1) and the untreated cells (THP-1) were set as control. Cells proliferation potential was assessed by MTT assay; flow cytometry was used to detect the cell cycle and cellular apoptosis. The mRNA and protein expression of BAX and BCL-2 were analyzed by RT-PCR and Western blot, respectively. Results Compared with the controls, growth ability of THP-1 mA cells was significantly improved ( P ＜ 0. 05 ). Additionally, the percentage of cells in the S phase increased significantly and that in the G, remarkably decreased ( P ＜ 0. 05 ). While there was no significantly change on cellular apoptosis and the expression of BAX and BCL-2 on the mRNA level or protein level. The ratio of BAX/BCL-2 also had no significantly change. Conclusions NPM1 mutations may promote cells proliferation potential in vitro, but had no significantly influence on cellular apoptosis.关键词
NPM1突变/白血病/THP-1细胞系/增殖/凋亡Key words
NPM1 mutations/leukemia/THP-1 cell line/proliferation/apoptosis分类
医药卫生引用本文复制引用
覃凤娴,邵会媛,陈先春,谭诗,张慧娟,肖玲,张伶..过表达突变的NPM1基因对THP-1白血病细胞增殖和凋亡的影响[J].基础医学与临床,2012,32(3):251-256,6.基金项目
国家自然科学基金(30872418) （30872418）
重庆市科委自然科学基金(CSTC2010BB5363) （CSTC2010BB5363）
