基础医学与临床2012，Vol.32Issue(3)：257-260,4.
低氧无血清对乳鼠心肌细胞皮肤桥蛋白表达的影响
Effect of hypoxia and serum deprivation on the expression of dermatopontin in cultured neonatal rat cardiomyocytes
摘要
Abstract
Objective To investigate the expression of dermatopontin ( DPT) induced by hypoxia and serum deprivation (H/SD) which simulated the microenvironment of ischemic myocardium in cultured neonatal rat cardiomyo-cytes in vitro. Methods Cultured neonatal rat cardiomyocytes were disposed to H/SD for 0, 6, 12 and 24 h. The mRNA and protein expression of DPT was determined by real time RT-PCR and western blot, respectively. Using enzyme-linked immunosorbent assay ( ELISA) to determine the different expression of DPT between normal cultured cardiomyocytes and cardiomyocytes disposed to H/SD for 24 h. Results Compared the data of 0 h, the mRNA and protein level of DPT were significantly raised in cultured cardiomyocytes which were disposed to H/SD for 6,12 and 24 h (P＜0. 05). It was confirmed by the method of ELISA that compared with control group, the expression of DPT was higher in cardiomyocytes which were disposed to H/SD for 24 h. Conclusions The DPT expression in cultured neonatal rat cardiomyocytes was promoted by H/SD in vitro, which suggested that DPT may be invoved in the ventricular remolding process caused by hypoxia and ischemic in vivo.关键词
皮肤桥蛋白/低氧无血清/心肌细胞Key words
dermatopontin/hypoxia and serum deprivation/cardiomyocyte分类
医药卫生引用本文复制引用
刘晓艳,张秀芳,魏英杰,史强,崔传珏,白媛媛..低氧无血清对乳鼠心肌细胞皮肤桥蛋白表达的影响[J].基础医学与临床,2012,32(3):257-260,4.基金项目
高等学校博士学科点专项科研基金(20091106110018) （20091106110018）
国家高技术研究发展计划(863计划)(2006AA02Z4B8) （863计划）
