基础医学与临床2012，Vol.32Issue(3)：283-290,8.
小鼠骨髓间充质干细胞与脑胶质细胞及基底膜细胞共培养后向毛细胞样细胞分化
Differentiation of bone marrow mesenchymal stem cells into hair like cells by co-culture with astrocytes and basement membrane cells of mice
摘要
Abstract
Objective To establish a method of efficient production of hair like cells from bone marrow mesenchy-mal stem cells (MSCs) in vitro. Methods MSCs、astrocytes( ASTs) and basement membrane cells (BAMCs) were isolated from mice, passed and depurated into third generation. We co-cultured MSCs with astrocytes (ASTs) in DMEM/F12/RA + 10% FBS for 7 days,then checked the surface molecule of Nestin by immunocytochemical analyses. Afterward, These cells were co-cultured with BAMCs for 14 days in DMEM/F12 + 10% FBS. Then checked the expression of hair cell markers ( MyosinⅦa and Mathl) by immunocytochemical analyses. We checked the mRNA expression of hair cell markers ( Myosin Ⅶa and Mathl) by RT-PCR. Results MSCs which were co-cultured with ASTs for 7 days expressed Nestin. Then Nestin-positive cells co-cultured with BAMCs for 14 days could express mRNA and protein of hair cell markers ( Mathl and MyosinVIIa). Conclusions MSCs co-cultured with ASTs and BAMCs could be induced to differentiate into hair like cells.关键词
骨髓间充质干细胞/毛细胞/共培养Key words
mesenchymal stem cells/hair cells/co-cultured分类
医药卫生引用本文复制引用
杨文飞,黄建民..小鼠骨髓间充质干细胞与脑胶质细胞及基底膜细胞共培养后向毛细胞样细胞分化[J].基础医学与临床,2012,32(3):283-290,8.基金项目
福建省科技厅、科技合作重点项目(2008I0017) （2008I0017）
