基础医学与临床2012，Vol.32Issue(3)：327-331,5.
磁性纳米粒子-抗体复合物分离肝癌线粒体
Isolation of mitochondria from liver cancer tissue with nanoparticle-antibody complex
摘要
Abstract
Objective To identify differences between magnetic nanoparticles-antibody complex and the traditional method to isolate mitochondria from human liver cancer tissues. Methods Using magnetic nanoparticles-antibody complex and density gradient centrifugation to isolate mitochondria of human liver cancer tissues separately, then scanning electron microscopy was used to scan the morphology of mitochondria, Western blot was used to detect mi-tochondrial protein markers and flow cytometry was used to analyze the alteration of mitochondrial membrane potential. Results The mitochondria isolated by magnetic nanoparticle-antibody had intergral structure, contained almost membrane no contaminant of nucleus, cell membrane, peroxisomes and lysosomes. The mitochondrial potential was stable. Conclusions Magnetic nanoparticle-antibody complex method gets more advantages, such as the preparation is easier, the structure of mitochondria is intact, the purity and activity of mitochondria are higher, expensive equipments are not needed.关键词
磁性纳米粒子-抗体复合物/密度梯度离心/线粒体/分离纯化Key words
magnetic nanoparticles-antibody complex/density gradient centrifugation/mitochondria/purification分类
医药卫生引用本文复制引用
唐博,梁锐,张易,金学利,赵樑,王立明..磁性纳米粒子-抗体复合物分离肝癌线粒体[J].基础医学与临床,2012,32(3):327-331,5.基金项目
国家高技术研究发展计划(863计划)(2006AA02A309) （863计划）
