极地研究2011，Vol.23Issue(4)：283-288,6.DOI:10.3724/SP.J.1084.2011.00283
低温脂肪酶基因在巴斯德毕赤酵母中的高效表达
HIGH-LEVEL EXPRESSION OF A COLD-ADAPTIVELIPASE GENE IN PICHIA PASTORIS
摘要
Abstract
A cold-adaptive lipase gene Zip-948 from Antarctic psychrophilic bacteria was ligated into the expression vector Ppic9k, which was subsequently transformed into yeast GS115. A high-level of yeast expression was observed. Lipase activity of the supernatant reached 27.5 U ? Ml-1 after 48 h fermentation, which is higher than that for most other low-temperature bacteria lipase genes expressed in Pichia pastoris.关键词
低温脂肪酶表达载体pPIC9K/巴斯德毕赤酵母高效表达Key words
low-temperature lipase, pPIC9K, Pichia pastoris, high-level expression引用本文复制引用
王永杰,沈继红,丛柏林,林学政,黄晓航..低温脂肪酶基因在巴斯德毕赤酵母中的高效表达[J].极地研究,2011,23(4):283-288,6.基金项目
863计划"极地特殊海洋微生物资源利用的关键技术研究(2007 AA091905)"资助 （2007 AA091905）
